Problemas
12. Which molecular biology tool is used to make multiple copies of DNA sequences? A. DNA ligase B. Restriction enzymes d. Pelymerase Chain Reaction (PCR) (b. Plasmids
Roztwór
Armando
maestro · Tutor durante 5 años
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(231 Votos)
Respuesta
The correct answer is C. Polymerase Chain Reaction (PCR).Polymerase Chain Reaction (PCR) is a molecular biology technique used to make multiple copies of a specific DNA sequence. It involves the use of a DNA polymerase enzyme to amplify a target DNA sequence, resulting in the production of millions to billions of copies of that sequence.The PCR process involves three main steps: denaturation, annealing, and extension. During denaturation, the DNA sample is heated to separate the double-stranded DNA into single strands. In the annealing step, short single-stranded DNA sequences called primers bind to their complementary sequences on the single-stranded DNA. Finally, in the extension step, the DNA polymerase enzyme adds nucleotides to the primers, synthesizing new strands of DNA that are complementary to the original strands.DNA ligase, restriction enzymes, and plasmids are all important tools in molecular biology, but they are not used to make multiple copies of DNA sequences. DNA ligase is an enzyme that joins two DNA fragments together, restriction enzymes cut DNA at specific sequences, and plasmids are small circular DNA molecules found in bacteria.