Problemas
Put the steps of PCR in the correct sequence. - annealing - extension - denaturation
Roztwór
Susana
maestro · Tutor durante 5 años
3.7
(121 Votos)
Respuesta
The correct sequence of steps in PCR is: Denaturation, Annealing, Extension.
Explicación
Polymerase Chain Reaction (PCR) is a technique used in molecular biology to make several copies of a specific DNA segment. The process involves three main steps: denaturation, annealing, and extension.## Step 1: DenaturationIn this step, the DNA sample is heated to around 94-98°C. This high temperature causes the double-stranded DNA to separate into two single strands.## Step 2: AnnealingAfter denaturation, the temperature is lowered to around 50-65°C. This allows the primers to bind to their complementary sequences on the single-stranded DNA.## Step 3: ExtensionIn the final step, the temperature is raised to around 72°C. This is the optimal temperature for the DNA polymerase to synthesize new strands of DNA by adding nucleotides to the primers.