4. Compare the amino acid sequence of human cytochrone C with that of the other 8 vertebrates. For each vertebrate,count the number of amino acids that differ from those in the human and write the number in the table to the right. 5. Based on this information which vertebrate do you think is most closely related to humans and why? 6. Based on this information, which vertebrate do you think is least related to humans and why? 7. If fossils were found in Layer C (sandstone) and Layer A (slate and siltstone ), which fossil would be older and why?

4. To compare the amino acid sequence of human cytochrome C with that of other vertebrates, you would need to align the sequences and count the differences. This requires access to the specific sequences for each vertebrate.<br /><br />5. The vertebrate most closely related to humans would be the one with the fewest differences in the amino acid sequence of cytochrome C compared to humans. This is because fewer differences indicate a closer evolutionary relationship.<br /><br />6. Conversely, the vertebrate least related to humans would be the one with the most differences in the amino acid sequence of cytochrome C compared to humans. More differences suggest a more distant evolutionary relationship.<br /><br />7. If fossils were found in Layer C (sandstone) and Layer A (slate and siltstone), the fossil in Layer A would be older. This is based on the principle of superposition in geology, which states that in undisturbed layers of rock, the oldest layers are at the bottom and the youngest are at the top. Therefore, since Layer A is below Layer C, it would contain the older fossil.