Put the steps of PCR in the correct sequence. - annealing - extension - denaturation

Polymerase Chain Reaction (PCR) is a technique used in molecular biology to make several copies of a specific DNA segment. The process involves three main steps: denaturation, annealing, and extension.<br /><br />## Step 1: Denaturation<br />In this step, the DNA sample is heated to around 94-98°C. This high temperature causes the double-stranded DNA to separate into two single strands.<br /><br />## Step 2: Annealing<br />After denaturation, the temperature is lowered to around 50-65°C. This allows the primers to bind to their complementary sequences on the single-stranded DNA.<br /><br />## Step 3: Extension<br />In the final step, the temperature is raised to around 72°C. This is the optimal temperature for the DNA polymerase to synthesize new strands of DNA by adding nucleotides to the primers.